miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury
miR-214-3p regulates microglial pyroptosis in WMI mice. 40 mg/kg agomiR-214-3p was injected into the WMI mouse model i.p. Microglia were isolated with a CD11b cell separation kit. (a) Dual-color staining for lba1 and caspase-1 in the white matter. (b) Immunoblot for procaspase-1 P20. (c) Relative protein level quantification by normalizing with β-actin. (d) FLICA assay staining for caspase-1 (representative dot plot). (e) FLICA assay staining for caspase-1 (statistical data). (f) IL-1β release after 24 hours of culture ex vivo. (g) IL-18 released after 24 hours of culture ex vivo. Data are . Individual data points are displayed: , , and .