Knocking Out of CEACAM1 Can Reduce Oxidative Stress and Promote Cell Proliferation in the HPMVECs under Hypoxia

Figure 2

HPMVECs infected with mutated CEACAM construct exhibit reduced CEACAM protein expression. (a) Quantification of CEACAM1 expression in control and mutant HPVMECs. (b) CEACAM1 knockout was confirmed by DNA sequencing. The mutation is highlighted by a red box. (c) CEACAM1 mutant cells exhibit reduced CEACAM protein expression compared to control HPMVECs by Western blot. (d) shows that successful constructions of CEACAM1^-/- and negative control stably transfected cell lines.

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