Conciliatory Anti-Allergic Decoction Attenuates Pyroptosis in RSV-Infected Asthmatic Mice and Lipopolysaccharide (LPS)-Induced 16HBE Cells by Inhibiting TLR3/NLRP3/NF-κB/IRF3 Signaling Pathway

Figure 7

Effect of the conciliatory anti-allergic decoction on the levels of pyroptosis and TLR3/NLRP3/NF-κB/IRF3 pathway-related proteins in the lungs of mice with OVA/RSV-induced asthma. (a) The lung tissue viral load was measured using a qPCR assay. (b) The mRNA levels of TLR3, NF-κB, and IRF3 in lung tissues. (c) Western blot detected the expression of Bax, Bcl-2, and Cleaved caspase-3 in lung tissues. (d, e) The pyroptosis-related protein expressions were analyzed by western blotting. (f) The TLR3/NLRP3/NF-κB/IRF3 pathway-related protein levels were also determined by western blotting. P < 0.05, P < 0.01, compared with the MC group.

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