Conciliatory Anti-Allergic Decoction Attenuates Pyroptosis in RSV-Infected Asthmatic Mice and Lipopolysaccharide (LPS)-Induced 16HBE Cells by Inhibiting TLR3/NLRP3/NF-κB/IRF3 Signaling Pathway

Figure 9

Effect of conciliatory anti-allergic decoction-contained serum on the cell survival of LPS-induced 16HBE cells. (a, b) The cell viability of 16HBE cells treated with LPS or CAD-contained serum was measured by using CCK-8 assays. (c) Cell viability of LPS-induced 16HBE cells treated with CAD-contained serum was evaluated by CCK-8 assay. (d) Representative flow cytometry results showing that the effects of CAD-contained serum on cell apoptosis in the LPS-induced 16HBE cells. (e, f, g, h) Western blotting was used to detect the expression of the apoptosis-, pyroptosis-, and TLR3/NLRP3/NF-κB/IRF3 pathway-associated proteins, Bax, Bcl-2, Cleaved caspase-3, NLRP3, ASC, Cleaved caspase-1, GSDMD, GSDMD-N, IL-18, IL-1β, TLR3, p-P65, P65, p-IκBα, IκBα, and IRF3 in LPS-induced 16HBE cells after treatment of CAD-contained serum. P < 0.05, P < 0.01.

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