miR-181a-5p Inhibits Pyroptosis in Sepsis-Induced Acute Kidney Injury through Downregulation of NEK7

Figure 6

miR-181a-5p inhibits pyroptosis of the LPS-induced HK-2 cells through downregulation of NEK7. (a) Morphology of LPS-induced HK-2 cells transfected with four vectors under an optical microscope (200x). The red arrow indicates pyroptosis of the cell. (b) Cell apoptosis of LPS-induced HK-2 cells transfected with four vectors by TUNEL, and (c) relative intensity of fluorescence. Scale . (d) Cell viability of LPS-induced HK-2 cells transfected with four vectors by CCK-8 assay. (e) The protein expression of NEK7, NLRP3, N-GSDMD, and active caspase-1 by Western blot, and (f) quantification by ImageJ. (g) The expression of inflammatory factors including IL-1β and IL-18. , .

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