Highly Expressing SCARA5 Promotes Proliferation and Migration of Esophageal Squamous Cell Carcinoma

Figure 2

Expression of SCARA5 in the ESCC cells and its impact on cellular proliferation and migration in vitro. (a) Expression of SCARA5 at mRNA level in the Eca109, EC9706, and TE-1 cells was detected using qRT-PCR. (b) Expression of SCARA5 at mRNA level in the Eca109 cells of the KD and the control groups was detected using qRT-PCR. (c) Expression of SCARA5 at protein level in the Eca109 cells of the KD and the control groups was detected using western blot. (d) Fluorescence field pictures of the Eca109 cells from the KD and the control groups were captured by Celigo imaging cytometer for constantly five days. (e–h) Effect of SCARA5 knockdown on proliferation of the Eca109 cells was evaluated via MTT assay. (i–k) Effect of SCARA5 knockdown on proliferation of the Eca109 cells was evaluated by clone formation assay. (l and m) Effect of SCARA5 knockdown on migration of the Eca109 cells was evaluated by wound healing assay. (n–p) Effect of SCARA5 knockdown on migration of the Eca109 cells was evaluated by cell invasion assay. (q and r) Effect of SCARA5 knockdown on cell cycle of Eca109 cells was evaluated by FCM. (s and t) Effect of SCARA5 knockdown on apoptosis of Eca109 cells was evaluated by FCM. (u–x) Effect of SCARA5 knockdown on proliferation of the EC9706 and TE-1 cells was evaluated via MTT assay. .

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