Highly Expressing SCARA5 Promotes Proliferation and Migration of Esophageal Squamous Cell Carcinoma

Figure 5

Effect of SCARA5 overexpression on the proliferation and migration of Hep 3B and Huh-7. (a–c) Expression of SCARA5 at mRNA level in Hep 3B and Huh-7 cells was detected by qRT-PCR before and after transfection of siRNA. (d and g) Expression of SCARA5 at protein level in Hep 3B and Huh-7 cells from the OE and the control group was detected by western blot assay. (e, f, h, and i) Effect of SCARA5 overexpression on the proliferation of Hep 3B and Huh-7 cells was evaluated using clone formation assay. (j–m) Effect of SCARA5 overexpression on the apoptosis of Hep 3B and Huh-7 cells was evaluated by FCM. (n–s) Effect of SCARA5 overexpression on the migration of Hep 3B and Huh-7 cells was evaluated by Transwell cell migration assay. .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)

(o)

(p)

(q)

(r)

(s)