The Effects of 6 Common Antidiabetic Drugs on Anti-PD1 Immune Checkpoint Inhibitor in Tumor Treatment

Figure 3

Metformin inhibits colon tumor growth and has no effect on tumor immune responses to anti-PD1. (a) Schematic overview of experimental design combined for (b)–(k). (b–d) Tumor growth (b) and weight (c) from MC38 tumor-bearing C57BL/6 mice were shown (). (e–g) Tumor growth (e) and weight (g) from CT26 tumor-bearing on BABL/C mice were shown (). (h and i) Sections from MC38 subcutaneous tumors were stained with anti-CD8 antibody by immunohistochemistry (h), and CD8⁺ T cells infiltration were analyzed in (i). (j and k) sections from CT26 subcutaneous tumors were stained with anti-CD8 antibody by immunohistochemistry (j), and CD8⁺ T cells infiltration were analyzed in (k). Scale bars: 100 μm. Data were analyzed by two-way ANOVA (b and e) and -test (c, f, i, and k). ns: no significance; , , and . Error bars denote for the s.e.m.

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