Chinese Herbal Formula Huayu-Qiangshen-Tongbi Decoction Attenuates Rheumatoid Arthritis through Upregulating miR-125b to Suppress NF-κB-Induced Inflammation by Targeting CK2

Figure 5

HQT ameliorates LPS-induced inflammation through upregulating miR-125b to suppress NF-κB-induced inflammation by targeting CK2. (a) MH7A cells were treated with different concentrations of HQT such as 0, 0.1, 1, 10, and 100 mg/mL for 24 h; cell viability was evaluated via the CCK-8 assay. (b–e) MH7A cells were transfected with miR-125b mimics or miR-125b inhibitor and then treated with LPS and PDTC. (b) The mRNA expression of miR-125b, pre-miR-125b, and pri-miR-125b was evaluated via qRT-PCR. (c) The protein level of p-p65 and p-IkB-α was evaluated via western blot. (d) Human CK2 concentration in the cultured supernatant was measured by using an ELISA assay. (e) The cytokine protein levels including TNF-α, IL-1β, and IL-6 in the cellular supernatant were measured by using an ELISA assay. , , , and .

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