Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1⁺ Hematopoietic Stem-Like Cells in Homeostasis and during Infection

Figure 3

ISM1⁺ cells have a phenotype of progenitor cells related to endothelial, mesenchymal, and hematopoietic cells in the lung. (a) Lung whole ISM1⁺ cells were selected based on its CD45, CD31, CD105, c-kit, and CD90 expression, and subpopulations are shown. HSPCs (ISM1⁺CD45⁺CD31^-CD105⁺c-kit⁺), MSCs (ISM1⁺CD45^-CD31^-CD105⁺CD90⁺), and EPCs (ISM1⁺CD45^-CD31⁺CD105⁺c-kit⁺) are depicted. (b) Cell proportions of each subpopulation are plotted. (c) Total events corresponding to each subpopulation are plotted. (d) MFI of ISM1⁺ cells within each subpopulation are shown. Data shown are the mean plus SEM, . Statistical analysis was performed by one-way ANOVA, , .