Deoxyschizandrin Inhibits the Proliferation, Migration, and Invasion of Bladder Cancer Cells through ALOX5 Regulating PI3K-AKT Signaling Pathway

Figure 4

Deoxyschizandrin inhibits the proliferation, migration, and invasion of bladder cancer cells by downregulating ALOX5 and affecting the downstream PI3K-Akt signaling pathway. (a and b) CCK-8 assay showed that negative control (NC) or sh-NC or Deoxyschizandrin or overexpression-ALOX5 or Deoxyschizandrin+overexpression-ALOX5 could regulate HT1376 cells’ proliferation. (c and d) Wound healing assay showed that negative control (NC) or sh-NC or Deoxyschizandrin or overexpression-ALOX5 or Deoxyschizandrin+overexpression-ALOX5 could regulate HT1376 cells’ migration. (e and f) Wound healing assay showed that negative control (NC) or sh-NC or Deoxyschizandrin or overexpression-ALOX5 or Deoxyschizandrin+overexpression-ALOX5 could regulate J82 cells’ migration. (g and h) Transwell assay showed that negative control (NC) or sh-NC or Deoxyschizandrin or overexpression-ALOX5 or Deoxyschizandrin+overexpression-ALOX5 could regulate HT1376 cells’ invasion. (i and j) Transwell assay showed that negative control (NC) or sh-NC or Deoxyschizandrin or overexpression-ALOX5 or Deoxyschizandrin+overexpression-ALOX5 could regulate J82 cells’ invasion. (k and l) BALB/c nude mice injected into HT1376 cells had smaller volume tumor xenografts with negative control (NC) or sh-NC or Deoxyschizandrin or overexpression-ALOX5 or Deoxyschizandrin+overexpression-ALOX5 treatment. (m) Ki-67 assay showed that negative control (NC) or sh-NC or Deoxyschizandrin or overexpression-ALOX5 or Deoxyschizandrin+overexpression-ALOX5 treatment by BALB/c nude mice injected into HT1376 cells. (n) HE stain revealed that negative control (NC) or sh-NC or Deoxyschizandrin or overexpression-ALOX5 or Deoxyschizandrin+overexpression-ALOX5 treatment by BALB/c nude mice injected into HT1376 cells. (o and p) Western blot analysis; the expression levels of AKT, PI3K, p-AKT, and P-PI3K were treated negative control (NC) or sh-NC or Deoxyschizandrin or overexpression-ALOX5 or Deoxyschizandrin+overexpression-ALOX5 in HT1376 and J82 cells. Data represent . and ^# compared with Deoxyschizandrin+overexpression-ALOX5.

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