The Contribution of Hepatic Macrophage Heterogeneity during Liver Regeneration after Partial Hepatectomy in Mice

<div>The functions of activated macrophages were determined after PHx. (a, b) C57BL/6 mice were sacrificed at D2 or D4 after 2/3 PHx or sacrificed immediately after sham operation. Liver sections were stained with (a) anti-F4/80 and anti-CD86 antibodies or (b) anti-F4/80 and anti-CD206 immunofluorescence and counterstained with Hoechst. (c, d) The percentage of double-positive cells in macrophages were determined and quantitatively compared in (a, b). (e) F4/80<sup>+</sup> macrophages were sorted by MACS from HNPCs, and the mRNA levels of CCL2, IL-6, TNF-<i>α</i>, VEGF-A, MMP-2, and MMP-9 were determined by qRT-PCR. <span class="nowrap"><svg height="9.10327pt" id="M16" style="vertical-align:-0.3499298pt" version="1.1" viewbox="-0.0498162 -8.75334 100.757 9.10327" width="100.757pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M372 352C427 368 507 407 507 502C507 548 486 592 451 616C420 638 376 650 301 650H44V622C124 616 128 610 128 527V123C128 40 122 34 36 28V0H257C336 0 402 12 453 40C512 72 548 124 548 191C548 291 471 335 372 350V352ZM213 362V558C213 591 216 602 226 608C235 614 258 618 282 618C377 618 415 558 415 490C415 406 369 362 260 362H213ZM213 328H258C380 328 450 283 450 181C450 76 377 35 296 34C231 34 213 53 213 125V328Z"></path></g><g transform="matrix(.013,0,0,-0.013,7.761,0)"><path d="M433 39L423 65C413 59 399 54 387 54C370 54 352 69 352 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Journal of Immunology Research

fig3

Figure 3

Figure 3: The Contribution of Hepatic Macrophage Heterogeneity during Liver Regeneration after Partial Hepatectomy in Mice 