The Contribution of Hepatic Macrophage Heterogeneity during Liver Regeneration after Partial Hepatectomy in Mice

Figure 4

Liver regeneration was delayed when infiltration of circulating monocyte was blocked in CCR2^−/− mice after PHx. (a) C57BL/6 mice and CCR2-KO mice were sacrificed D1, 2, or 4 after 2/3 PHx or sacrificed immediately after sham operation. The subpopulation of HNPCs in control and CCR2-KO mice was analyzed by FACS with anti-F4/80, anti-CD11b, and anti-ly6C antibodies at the indicated times after PHx. (b) The ratios of KCs/IMs in (a) were determined and quantitatively compared. (c) Liver sections were stained with anti-Ki67 IHC. (d) The number of Ki67⁺ cells in (c) was counted and quantitatively compared. (e) Liver-body weight ratios of control and CCR2-KO mice were quantitatively compared at the indicated times. (f) Liver sections stained with anti-F4/80 and anti-Ki67 immunofluorescence and counter-stained with Hoechst. The arrow indicated the double-positive cells. (g) The number of F4/80⁺ cells in (f) was counted and quantitatively compared. (h) The percentage of double-positive cells in macrophages was determined and quantitatively compared in (f). , . , .

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