Helicobacter pylori Infection Elicits Type I Interferon Response in Human Monocytes via Toll-Like Receptor 8 Signaling

Figure 6

Phagocytic function of THP-1 macrophage is unaltered upon inhibition of both TLR7 and TLR8 mediated signaling. THP-1 cells were uninfected (mock), infected with H. pylori J99 strain, or infected and treated with TLR7 inhibitor (ODN20958 + J99) or TLR7/8 inhibitor (ODN20959 + J99), at MOI 50 for 4 hours or MOI 10 for 16 hours. Some cells were stripped off extracellular bacteria before analysis. Fluorescent intensity was examined by flow cytometrical analysis. (a) Histogram showing fluorescent intensity. (b) Bar charts showing the average percentages of cells containing FITC-labeled bacteria. Data is representative of three independent experiments.