Helicobacter pylori Infection Elicits Type I Interferon Response in Human Monocytes via Toll-Like Receptor 8 Signaling

Figure 8

Immunoblot analysis of TLR downstream signaling pathway molecules. THP-1 cells were infected with H. pylori J99 strain at MOI 10 for 16 hours. Cells were lysed and processed for immunoblot analysis with antibodies to phospho- or total IRF-3, IRF-7, IKKα/β, and β-actin as loading control. (a) Western blot data. (b) Bar charts showing average ratio of phosphorylated over total protein after normalizing to the β-actin level. Data is representative of two independent experiments. Statistical significance was analyzed using Student’s -test ().