Helicobacter pylori Infection Elicits Type I Interferon Response in Human Monocytes via Toll-Like Receptor 8 Signaling

Table 1

Primer sequences used in real-time qRT-PCR analysis.

Target	Forward primer sequence (5 to 3)	Reverse primer sequence (5 to 3)	NCBI accession no.
β-Actin	GGA CGA CAT GGA GAA AAT CTG GCA	ACT CGT CAT ACT CCT GCT TGC TG	NM_001101.4
TLR1	GCC AAA TGG AAC AGA CAA GCA	ACA GAT TCC TTT TGT AGG GGT GC	NM_003263
TLR2	GTT GTG GGT TGA AGC ACT GG	GGC TTG AAC CAG GAA GAC GA	NM_003264
TLR3	TCA GAA GAT TAC CAG CCG CC	TGA AAA CAC CCT GGA GAA AAC TCT	NM_003265
TLR4	CAG AAT GCT AAG GTT GCC GC	TTA GGA ACC ACC TCC ACG C	NM_138554
TLR5	TTT CAG GAG CCC GAG CGA	ATA GCA TCC CTG GTT TGG TGA C	NM_003268
TLR6	ATG ACC AAA GAC AAA GAA CCT ATT G	AAT TCC TTA CAG ATG GGC AGG	NM_006068
TLR7	ACT CCA TGC CAT CAA GAA AGT TGA	TCT GTG CAG TCC ACG ATC AC	NM_016562
TLR8	GAA ACA TGG TTC TCT TGA CAC TTC A	CTG GTG CTG TAC ATT GGG GT	NM_016610
TLR9	GAA GGG GTG AAG GAG CTG TC	CGG CAG AAA CCC ATG CTG	NM_017442
TLR10	AAG CCG TGG GAA TTC AGC AG	TGC CAT GTC TCC GTT GTT GA	NM_030956
IL-1β	ATG CAC CTG TAC GAT CAC TG	ACA AAG GAC ATG GAG AAC ACC	NM_000576.3
IL-6	CCA CTC ACC TCT TCA GAA CG	CAT CTT TGG AAG GTT CAG GTT G	NM_000600.5
TNF-α	ACT TTG GAG TGA TCG GCC	GCT TGA GGG TTT GCT ACA AC	NM_000594.4
IFN-α	GAC TCC ATC TTG GCT GTG A	TGA TTT CTG CTC TGA CAA CCT	NM_024013.3
IFN-β	TGC TCT CCT GTT GTG CTT CTC	CAT AGA TGG TCA ATG CGG CG	NM_002176.4