Circular RNA RHOT1 Regulates miR-142-5p/CCND1 to Participate in Chondrocyte Autophagy and Proliferation in Osteoarthritis

<div>CircRHOT1 is able to enhance the CCND1 expression by sponging miR-142-5p in chondrocytes. (a) The potential interaction of miR-142-5p with circRHOT1 and CCND1 was predicted. (b)–(d) The chondrocytes were treated with miR-142-5p mimic. (b) The expression of miR-142-5p was measured by qPCR. (c) The luciferase activity of circRHOT1 was determined by luciferase reporter gene assay. (d) The luciferase activity of CCND1 3<svg height="11.4859pt" id="M61" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>UTR was analyzed by luciferase reporter gene assay. (e) The expression of miR-142-5p was tested by qPCR in chondrocytes treated with circRHOT1 shRNA. (f) The expression of CCND1 was detected by qPCR in chondrocytes treated with circRHOT1 shRNA and miR-142-5p inhibitor. <span class="nowrap"><svg height="9.10327pt" id="M62" style="vertical-align:-0.3499298pt" version="1.1" viewbox="-0.0498162 -8.75334 58.8493 9.10327" width="58.8493pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M861 0V28C774 35 771 41 768 147L759 509C756 612 762 614 851 622V650H681L449 149L221 650H57V622C148 613 153 609 144 479L130 271C123 166 117 123 111 88C104 46 85 34 26 28V0H259V28C192 35 169 42 167 90C166 130 166 173 170 256L185 541H187L411 7H431L675 555H679L683 147C683 41 680 35 598 28V0H861Z"></path></g><g transform="matrix(.013,0,0,-0.013,11.244,0)"><path d="M380 106C343 72 306 56 265 56C195 56 116 112 115 248C235 252 361 262 377 265C396 269 400 277 400 297C400 374 333 449 250 449H249C198 449 144 421 103 376S37 269 37 201C37 88 109 -12 232 -12C263 -12 332 6 395 84L380 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Journal of Immunology Research

fig5

Figure 5

Figure 5: Circular RNA RHOT1 Regulates miR-142-5p/CCND1 to Participate in Chondrocyte Autophagy and Proliferation in Osteoarthritis 