ZNF165 Is Involved in the Regulation of Immune Microenvironment and Promoting the Proliferation and Migration of Hepatocellular Carcinoma by AhR/CYP1A1

Figure 3

CYP1A1 in tryptophan signaling can be regulated by ZNF165. (a) ZNF165 knockdown was achieved in Bel7402 cells by transducing short hairpin RNA targeting ZNF165 (sh-ZNF165-1/2), and differentially expressed genes (DEGs) were analyzed using array assay. DEGs were applied for signaling pathway enrichment annotation. (b, c) ZNF165 knockdown or overexpression was achieved in Bel7402 and HCCLM3 cells by transducing sh-ZNF165-1/2 or ZNF165-overexpressing plasmid (OE-ZNF165), and the mRNA expression of the tryptophan pathway-related genes AhR, CYP1A1, and CYP1B1 was determined using qRT-PCR.

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