Membrane Bound CRT Fragment Accelerates Tumor Growth of Melanoma B16 Cell In Vivo through Promoting M2 Polarization via TLR4

Figure 1

Construction and characterization of the B16-EGFP and B16-tmCRT/39-272 cell lines. (a) Surface expression of CRT on B16-EGFP and B16-tmCRT/39-272 cells were examined by FACS and confocal laser scanning microscopy. (b) MTT assay of B16-EGFP and B16-tmCRT/39-272 cell proliferation. (c) Cell adhesion assay of B16-EGFP and B16-tmCRT/39-272 cells post-scratch. (d) Wound healing assay for examining B16-EGFP and B16-tmCRT/39-272 cell migration. The width of the wound was measured 12 h and 24 h after wounding. (e and f) Female C57BL/6 mice ( per group) were s.c. injected with B16-EGFP or B16-tmCRT/39-272 cells ( μl/mouse). The diameter of solid tumors at the injection sites was evaluated every 2 days thereafter for 18 days. (e) Tumor growth curves. (f) Tumor weights. The experiments were repeated three times. , P <0.01, , ns: not significant.

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