Membrane Bound CRT Fragment Accelerates Tumor Growth of Melanoma B16 Cell In Vivo through Promoting M2 Polarization via TLR4

Figure 3

Macrophage depletion or TLR4 KO reduced tumorigenesis and M2 polarization in B16-tmCRT/39-272-inoculated mice. (a–d) Female C57BL/6 mice () were s.c. injected with B16-EGFP or B16-tmCRT/39-272 cells ( μL/mouse), then i.p. injected with Lipo-Clod or Lipo-PBS (100 μL/mouse) every 3 days. (a) Tumor growth curves. (b) Tumor weights. (c) Ascites macrophages were collected from the mice 15 days postinoculation. The proportions of total macrophages (CD11b⁺F4/80⁺) were analyzed by FACS. (d) Tumor tissues were collected from mice 15 days postinoculation. The proportions of M2 macrophages (F4/80⁺CD206⁺) were analyzed by FACS. (e and f) Female C57BL/6 () or TLR4-KO C57BL/6 mice () were s.c. injected with B16-EGFP or B16-tmCRT/39-272 cells (5 × 10⁵/100 μL/mouse) and the diameter of solid tumors at the injection sites was evaluated every 3 days thereafter. (e) Tumor growth curves. (f) Tumor weights. The experiments were repeated three times. , , , ns: not significant.

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