Tumor-Derived Exosomal miR-29b Reduces Angiogenesis in Pancreatic Cancer by Silencing ROBO1 and SRGAP2

Figure 2

miR-29b-containing exosomes reduced HUVEC migration and tube formation. miR-29b mimics or mimic NCs were transfected into BxPC3 and AsPC-1 cells. Forty-eight hours later, both cell types were cocultivated with HUVECs for 24 h and then maintained in the presence or absence of GW4869 for an additional 48 h. (a) RT-qPCR analysis of miR-29b expression in HUVECs. HUVECs were stimulated in the coculture system with BxPC3 and AsPC-1 cells. After 48 h of stimulation, (b) VEGF content in the culture medium of HUVECs was assessed by ELISA. (c) Tube formation assays were conducted to determine HUVEC tube formation (100x). (d) Transwell migration assays were conducted to determine HUVEC migration (200x). and vs. NC; ^# and ^### vs. mimics. Experiments were repeated for three times and presented as .

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