Tumor-Derived Exosomal miR-29b Reduces Angiogenesis in Pancreatic Cancer by Silencing ROBO1 and SRGAP2

Figure 3

Exosomal miR-29b from PC cells enhanced HUVEC migration and tube formation. (a) RT-qPCR analysis of miR-29b expression in recipient HUVECs incubated with exosomes secreted from BxPC3 and AsPC-1 cells transfected with miR-29 mimics and also in recipient HUVECs treated with the miR-29b inhibitor or inhibitor NC. The parental HUVECs served as blank control cells. (b) Tumor formation assays were performed on HUVECs treated with exosomes, exosomes+miR-29 inhibitor, and exosomes+miR-29 inhibitor NC (100x). The parental HUVECs served as blank control cells. (c) ELISA detection of VEGF levels in HUVECs treated with exosomes, exosomes+miR-29 inhibitor, and exosomes+miR-29 inhibitor NC. The parental HUVECs served as blank control cells. (d) Transwell migration assays for determining the migration of HUVECs treated with exosomes, exosomes+miR-29 inhibitor, and exosomes+miR-29 inhibitor NC. The parental HUVECs served as blank control cells (200x). and vs. blank; ^## and ^### vs. exosomes+NC. Experiments were repeated for three times and presented as .

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