Tumor-Derived Exosomal miR-29b Reduces Angiogenesis in Pancreatic Cancer by Silencing ROBO1 and SRGAP2

Figure 5

Exosomal miR-29b reduced HUVEC migration and tube formation by downregulating ROBO1 and SRGAP2. (a) RT-qPCR analysis of miR-29b expression in recipient HUVECs incubated with exosomes secreted from BxPC3 and AsPC-1 cells transfected with ROBO1 or SRGAP2 overexpressing constructs. (b) RT-qPCR analysis of ROBO1 and SRGAP expression in recipient HUVECs that had been incubated with exosomes secreted from BxPC3 cells transfected with ROBO1 or SRGAP2 overexpression constructs. (c) Western blot assessment of SRGAP2 and ROBO1 protein expression in recipient HUVECs incubated with exosomes secreted from AsPC-1 cells transfected with ROBO1 or SRGAP2 overexpressing constructs. (d) ELISA detection of VEGF levels in HUVECs incubated with exosomes secreted from the AsPC-1 cells transfected with ROBO1 or SRGAP2 overexpressing constructs. (e) Tube formation assay to assess the tube formation ability of HUVECs incubated with exosomes secreted from AsPC-1 cells transfected with ROBO1 or SRGAP2 overexpressing constructs (100x). (f) Transwell migration assay to assess the migration ability of HUVECs incubated with exosomes secreted from AsPC-1 cells transfected with ROBO1 or SRGAP2 overexpressing constructs (200x). and vs. NC; ^#, ^##, and ^###, vs. mimics. Experiments were repeated for three times and presented as .

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