N-Glycosylation on Asn50 of SND1 Is Required for Glioma U87 Cell Proliferation and Metastasis

Figure 5

Elimination of N-glycans at Asn50 inhibits cell proliferation and metastasis. (a) The proliferation ability of glioma cells was detected by CCK8 assay performed on both wild-type SND1-EGFP and N50Q-EGFP overexpressing cells with a duration of 4 days. (b) The cell invasion assay was detected by transwell assay administrated in the wild-type SND1-EGFP and N50Q-EGFP overexpressing cells, and invaded cells were fixed and stained with 0.5% crystal violet. Scale bar: 100 μm. (c) Representative images of the bottom surface were captured using a phase-contrast microscopy (upper). The numbers of migrated cells in nine random microscopic fields (×200) were counted and averaged. Data represent the of three independent experiments. ; .

(a)

(b)

(c)