Peptidylarginine Deiminase 2 in Murine Antiviral and Autoimmune Antibody Responses

Figure 2

T cells in collagen-induced arthritis (CIA) are unaffected by loss of PAD2. Seventeen weeks after the first injection to induce CIA, splenocytes were harvested and stained for flow cytometry. Debris, clustered cells, and dead cells were excluded using forward scatter, side scatter, and a viability dye. (a) Percent of CD3+ splenocytes that are CD4+ and CD8+ ( mice, 3 experiments). Activation status of these CD3+ cells was evaluated with (b) representative plots as well as of (c) CD4+ and (d) CD8+ populations that are effector (CD62L-CD44+), memory (CD62L+CD44+), and naïve (CD62L+CD44-) shown. The percent of CD4+ cells that produce IL-17A, IL-4, and IFNγ was also quantified with (e) representative plots and (f) graphed ( mice, 3 experiments). For all panels, PAD2^+/+ versus PAD2^-/- data were compared by paired test, and no comparisons were significant.

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