HIF-3α-Induced miR-630 Expression Promotes Cancer Hallmarks in Cervical Cancer Cells by Forming a Positive Feedback Loop

Figure 4

RNA-seq revealed the functions of miR-630 targets in HeLa cells. (a) Read distribution plot showed the significant HIF3A binding density in the promoter region of miR-630 host gene. Red frame represented the HIF3A binding peak. (b) Bar plot showed the ChIP-qPCR results of the HIF-3a binding density in the promoter region of miR-630 host gene. (c) Western blot showed the expression changes of HIF1A and HIF3A upon miR-630 overexpression and knockdown. (d) Bar plot showed the quantified levels of WB results shown in (c). Three biological replicates were included in this panel. (e) Bar plot showed the enriched GO BP terms for upregulated genes (left) and downregulated genes (right) by miR-630 inhibition. (f) Bar plot showed the increased expression levels of ATM and ATR by miR-630 inhibition. (g) Bar plot showed the increased expression level of HIF1A and the decreased expression level of HIF3A by miR-630 inhibition.

(a)

(b)

(c)

(d)

(e)

(f)

(g)