HIF-3α-Induced miR-630 Expression Promotes Cancer Hallmarks in Cervical Cancer Cells by Forming a Positive Feedback Loop

Figure 5

miR-630 increases the radioresistance and significantly reduces the apoptosis level of HeLa cells under spontaneous and radiation-treated conditions. (a) RT-qPCR analysis of lentiviral overexpression and inhibition of miR-630 in HeLa cell lines. (b) Colony formation assay showing the higher radioresistance by miR-630 overexpression and opposite phenotype by miR-630 inhibition. . (c, d) Overexpression of miR-630 increased the cell proliferation rates of HeLa cells under irradiation conditions. (e) miR-630 overexpression reduced the spontaneous apoptosis levels of HeLa cells. (f, g) miR-630 overexpression reduced the radiation-induced apoptosis levels of HeLa cells. (h) Western blotting showing the expression changes of several apoptosis markers in HeLa cells with miR-630 overexpression or inhibition under radiation-treated conditions. (i) Western blotting showing the expression changes of several apoptosis markers in HeLa cells with miR-630 overexpression or inhibition under spontaneous conditions.

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