HIF-3α-Induced miR-630 Expression Promotes Cancer Hallmarks in Cervical Cancer Cells by Forming a Positive Feedback Loop

Figure 6

Effects of miR-630 on migration and invasion of HeLa cell line and miR-630 promoted HeLa metastasis is mediated by the EMT. (a) Wound-healing assay was performed to compare the migratory capabilities of HeLa, HeLa-virus control, and HeLa-miR-630-inhibitor cells. . The graph shows the quantification of migration rates analyzed in HeLa, HeLa-virus control, HeLa-miR-630, and HeLa-miR-630-inhibitor cells, respectively. (b, c) Representative invasion images of HeLa, HeLa-virus control, HeLa-miR-630, and HeLa-miR-630-inhibitor cells by transwell invasion assay. The right graph shows the quantification of invasion numbers analyzed in four cells. (d) Heat map plot showed the expression level changes of miR-630 targets that are transcription factors related to EMT. (e) Bar plot showed the RT-qPCR results of transcription factor EP300 upon miR-630 overexpression and knockdown. (f) The morphology of HeLa, HeLa-virus control, HeLa-miR-630, and HeLa-miR-630-inhibitor cells. Note: (A) cell space became wide; (B) spindle-cell morphology formed; (C) pseudopodia were stretched out. . (g) WB was performed to analyze the expression of EMT makers. (h) Cellular staining showed the cytokeratin distribution in the HeLa cells. (i) Working model showed the HIF3A-miR-630 regulatory loop in HeLa cells.

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