Semaphorin-3 Promotes Specific Immunotherapy Effects on Experimental Food Allergy

Figure 1

Pak1 is correlated with mast cell apoptosis resistance. (a–c) Mast cells were isolated from the mouse intestine and analyzed using RT-qPCR and Western blotting: (a) Pak1 mRNA levels; (b) Pak1 protein levels; (c) integrated density of Pak1 blots in (b). (d) Mast cell frequency in LPMCs (lamina propria mononuclear cells). (e) Positive correlation between Pak1 mRNA levels in mast cells and mast cell frequency in LPMCs. (g, h) Mast cells were isolated from LPMCs and exposed to C48/80 in the culture overnight. Gated FCM plots show apoptotic mast cell counts. Boxplots show summarized apoptotic mast cell counts. (i) Negative correlation between Pak1 mRNA levels in mast cells and the apoptotic mast cell counts. Each bubble in boxplots presents data obtained from one sample. The data of (b) are from one experiment that represents 3 independent experiments with pooled protein extracts from mast cells obtained from 10 experiments. NC: naïve control mice.

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