Semaphorin-3 Promotes Specific Immunotherapy Effects on Experimental Food Allergy

Figure 2

Assessment of the role of sensitization in Pak1 expression and apoptosis development in mast cells. (a, b) BMMCs were sensitized (Sens, in short) by exposing to OVA-specific IgE in the culture overnight. Violin plots show Pak1 mRNA levels, and immunoblots show Pak1 protein levels in BMMCs. (c) Integrated density of Pak1 blot density. (d, e) Sensitized BMMCs were stimulated with cisplatin (20 μM) or C48/80 (0.1 mg/ml) in the culture overnight. Gated FCM plots show apoptotic BMMC cells. Boxplots show apoptotic BMMC frequency. (f) A negative correlation between Pak1 mRNA levels in sensitized BMMCs and the cisplatin-induced apoptotic BMMC frequency. Each bubble in the violin plots and boxplots presents data obtained from one experiment. (ANOVA+Dunnett’s test), compared with the NC (naïve control) group. NC: Naïve control BMMCs.

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