Semaphorin-3 Promotes Specific Immunotherapy Effects on Experimental Food Allergy

Figure 6

Assessment of sema3A effects on promoting AIT. FA mice were treated with sema3A (Sem, in short) or/and AIT or PBS. (a) Diarrhea mice during 2 h after allergen challenge. (b) Core temperature changes at 30 min after allergen challenge. (c) Serum allergen-specific IgE (sIgE) levels. (d–f) Gut lavage fluid (GLF) Th2 cytokine levels. (g, h) GLF EPO and mMCP1 levels. (i) Gated plots show mast cell frequency in LPMCs. (j) Summarized mast cell frequency in LPMCs. (k–m) RAS activation status in mast cells of the intestine. The data of boxplots are presented as median (IQR). (ANOVA+Dunnett’s test) compared with the naïve control (NC) group. ^!!! ( test), compared with the PBS group. ^### ( test), compared with the AIT alone group. Each bubble presents data obtained from one mouse. The data of (i) are from one experiment that represent 6 independent experiments.

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