Research Article
Diet-Induced Obesity Promotes Liver Metastasis of Pancreatic Ductal Adenocarcinoma via CX3CL1/CX3CR1 Axis
Figure 5
CX3CL1 promoted recruitment of CX3CR1-positive pancreatic tumor cells. (a) mRNA levels of CX3CR1 in PDAC cells. (b) Western blot analysis of CX3CR1 in human PDAC cell lines. (c) Flow cytometry analysis of the surface expression level of CX3CR1 in different human cell lines. (d)The expression level of soluble CX3CL1 was examined by using ELISA. Protein samples were obtained from the mouse liver. (e) Effect of CX3CL1 on the migratory ability of mouse PDAC cell lines Panc02; relative transferred cell numbers were analyzed. Cell migration experiments in blocker group were performed by using the blocking antibody of CX3CL1. The neutralization dose (ND50) is 1 μg/ml in the presence of 30 ng/ml mouse recombinant CX3CL1. Scale bar 10 μm. (f) Effect of CX3CL1 on the migratory ability of human PDAC cell lines AsPC-1 and Capan-1. Different concentration of CX3CL1 recombinant protein was used in cell transwell experiments; relative transferred cell numbers were analyzed. The neutralization dose (ND50) is 3 μg/ml in the presence of 100 ng/ml human recombinant CX3CL1. Scale bar 10 μm. , , , and .
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