MZF1 Transcriptionally Activated MicroRNA-328-3p Suppresses the Malignancy of Stomach Adenocarcinoma via Inhibiting CD44

Figure 4

miR-328-3p worked as a cancer suppressor in STAD via targeting CD44. To further indicate whether miR-328-3p regulated the behaviors of STAD cells in a CD44-dependent manner, AGS cells were cotransfected with miR-328-3p inhibitors and si-CD44. (a) qRT-PCR and Western blot were utilized to detect the transfection efficiency of CD44 knockdown. (b) CCK-8 assay was used to assess the proliferation rate of AGS cells. (c) Western blot assay was used to detect the expression levels of apoptosis-related proteins. (d, e) The migration and invasion of AGS cells were assessed with (d) wound healing and (e) Transwell assays. (f) The expression levels of SOX2, NANOG, EPCAM, CD133, and CD166 were determined by qRT-PCR.  μm. All experiments were performed in triplicate. , , and .

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