MZF1 Transcriptionally Activated MicroRNA-328-3p Suppresses the Malignancy of Stomach Adenocarcinoma via Inhibiting CD44

Figure 5

MZF1 activated the transcription of miR-328-3p. (a) PuTmiR and TransmiR databases were used to predict the transcription factor of miR-328-3p. MZF1, RREB1, BACH1, and STAT1 were four common transcription factors. (b) The expression levels of indicated four transcription factors in STAD tissues were analyzed. (c) The analysis of GC dataset in Oncomine database revealed MZF1 expression levels in STAD tissues. (d) qRT-PCR detection of MZF1 expression in STAD cells. (e, f) qRT-PCR assay was utilized to detect the expression levels of MZF1, miR-328-3p, and CD44 in (e) transfected AGS and (f) SNU16 cells. (g) Western blot assay was employed to detect the expression levels of MZF1 and CD44. (h) The TransmiR database was used to predict the binding sites of MZF1 in pre-miR-328-3p promoter regions. (i, j) The interaction between MZF1 and miR-328-3p was confirmed by (i) ChIP and (j) dual-luciferase reporter assays. All experiments were performed in triplicate. , , and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)