HIF-1α Regulated WTAP Overexpression Promoting the Warburg Effect of Ovarian Cancer by m6A-Dependent Manner

Figure 6

WTAP-dependent m6A demethylation regulates the processing of miR-200 via DGCR8. (a) MiR-200 was detected using RT-qPCR upon WTAP depletion or overexpression in A2780 and 3AO cells. (b) MiR-200 was quantified using RT-qPCR upon WTAP overexpression in H8901 and OVCAR8 cells. (c and d) pri-miR200 was detected by RT-qPCR upon WTAP depletion or overexpression in A2780/3AO and H8901/OVCAR8 cells. (e) Immunoprecipitation of DGCR8-associated RNA from control or WTAP-overexpressing cells followed by RT-qPCR to detect pri-miRNA binding to DGCR8. Pri-let-7e was used as a positive control. Three independent experiments were performed. (f) Immunoprecipitation of m6A modified RNA in control or WTAP-overexpressing cells followed by RT-qPCR to estimate the m6A modification levels of pri-miRNAs. IgG = immunoglobulin G; IP = immunoprecipitation; oe = overexpressing).

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