A Novel Prognostic Signature Revealed the Interaction of Immune Cells in Tumor Microenvironment Based on Single-Cell RNA Sequencing for Lung Adenocarcinoma

Figure 3

The mining of the hub module in WGCNA analysis. Determination of key module and key cluster in LUAD. (a) The cell abundance of the 23 clusters in bulk RNA seq of the TCGA-LUAD normal and tumor samples. (b) The sample clustering plot to remove outlier samples. (c) The scale-free fit index for various soft-thresholding powers. (d) dendrogram based on different metrics before and after merging small modules. (e) The nine modules, with the number in parentheses, represent the transcript number. Each row corresponds to a module, and each column corresponds to a cluster cell abundance. The color scale on the right shows module-trait correlations from 1 (red) to −1 (blue). Each cell at the row-column intersection’s background color represents the correlation coefficient between the modules and clusters. The red color indicates a high degree of positive correlation, and the blue indicates a high degree of negative correlation between each module and the clusters. Each cell also contains the corresponding signatures (bottom symbols).

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