Research Article
BACH2 in TRegs Limits the Number of Adipose Tissue Regulatory T Cells and Restrains Type 2 Immunity to Fungal Allergens
Figure 1
Bach2 regulates classical and regulatory T cell homeostasis. Splenocytes from naïve WT and Bach2loxp/loxpFoxP3-Cre mice were stained for intracellular and extracellular antigens and analyzed by flow cytometry. (a) Frequency and (b) number of Foxp3+ve Treg cells. (c) Histograms are gated on Foxp3+ve Treg cells and show staining for the indicated markers in WT (shaded) and Bach2loxp/loxpFoxP3-Cre mice (line) mice. (d) Frequency and numbers of Tregs expressing the indicated molecules. (e) Activation of classical T cells was analyzed by staining with anti-CD44 and anti-CD62L; contour plots are gated on CD4 or CD8 T cells. (f), (g) Splenocytes were stimulated for 5 hrs in vitro with PMA and ionomycin in the presence of Brefeldin A, and cytokine production by CD4 and CD8 T cells was measured by intracellular staining for (f) IFN-γ and (g) IL-13 and IL-17A. This experiment was repeated three times with similar results, 4-5 mice per group. .
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