BACH2 in TRegs Limits the Number of Adipose Tissue Regulatory T Cells and Restrains Type 2 Immunity to Fungal Allergens

Figure 4

Bach2-deficiency in Tregs does not affect systemic or mucosal Type 1 immunity. WT and Bach2^loxp/loxpFoxP3-Cre mice were infected with (a-d) LCMV-Armstrong or (e-h) PR8/H1N1 influenza virus, and Tregs were analyzed by flow cytometry. (a) Frequency and numbers of splenic Tregs in LCMV-infected mice. (b) Frequency of effector Treg subsets in the spleen. (c) Frequency and numbers of tetramer binding CD8 (specific to NP396 or GP33 epitopes) and CD4 (specific for GP66 epitope) T cells in the spleen. (d) Splenocytes were stimulated for 5 hrs in vitro with LCMV peptides in the presence of Brefeldin A, and cytokine production was measure by intracellular staining for IFNγ in CD8 and CD4 T cells from the spleen. (e) Frequency and numbers of Tregs in the lung of influenza virus-infected mice. (f) Frequency of effector Treg subsets in the lung. (g) Frequency and numbers of influenza virus-specific CD8 (specific to NP66 and PA224 epitopes) and CD4 (specific to NP311 epitope) T cells in the lung. (h) Mononuclear cells from the lung were stimulated for 5 hrs in vitro with PR8 peptides or PMA and ionomycin in the presence of Brefeldin A, and cytokine production was measured by intracellular staining of IFNγ in CD8 and CD4 T cells from the lung. This experiment was repeated three times with similar results, 4-5 mice per group. .

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