Research Article
BACH2 in TRegs Limits the Number of Adipose Tissue Regulatory T Cells and Restrains Type 2 Immunity to Fungal Allergens
Figure 5
Bach2 is required for Tregs to control TH2 immunity in lungs. WT and Bach2loxp/loxpFoxP3-Cre mice were administered Aspergillus melleus fungal protease intratracheally and lungs were harvested on day 15; mononuclear cells from lungs were analyzed by flow cytometry. (a) Frequency and numbers of Foxp3+ve Tregs in the lungs. (b) Frequency of KLRG-1-, CXCR3-, CD103-, and ST2-positive Tregs in lungs. (c) MFI levels for BATF, IRF4, and GATA3 staining in Foxp3+ve Tregs. (d) Numbers of inflammatory cell subsets in the lungs. (e) Cells from the lung were stimulated for 6 hrs in vitro with heat-inactivated fungal protease in the presence of Brefeldin A during the last 4 hrs, and cytokine production was measure by intracellular staining of IFNγ, IL-5, IL-13, and IL17 in CD4 T cells. This experiment was repeated two times with similar results, 4-6 mice per group. .
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