Research Article
ATXN2-Mediated PI3K/AKT Activation Confers Gastric Cancer Chemoresistance and Attenuates CD8+ T Cell Cytotoxicity
Figure 2
High ATXN2 expression promoted chemoresistance in GC. (a, b) ATXN2 upregulation in SGC7901 cells and ATXN2 downregulation in SGC7901/5-FU cells were verified at the protein (a) and mRNA (b) levels. (c) SGC7901 cells overexpressing ATXN2 and SGC7901/5-FU cells with downregulated ATXN2 were treated with 5-FU (10 μg/mL). The apoptosis rate of the two cell lines was detected. (d) The IC50 of SGC7901 cells overexpressing ATXN2 and SGC7901/5-FU cells with downregulated ATXN2. (e) SGC7901/5-FU cells with downregulated ATXN2 or vector control were transplanted into nude mice, and the weight and volume of tumors were measured. (f) IHC analysis of Ki67 and cleaved caspase-3 expression. and .
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