LMX1B Activated Circular RNA GFRA1 Modulates the Tumorigenic Properties and Immune Escape of Prostate Cancer

Figure 1

Expression patterns of circGFRA1 in PCa. (a) Microarray analysis of four pairs of prostate cancer tissues. (b) Relative expression of circGFRA1 in PCa cell lines showing circGFRA1 expression was significantly upregulated in PCa cell lines compared to RWPE-1 cells (). (c) Schematic illustration of circGFRA1. (d and e) The effect of ActD treatment on the stability of circGFRA1 and linear form GFRA1 in PC-3 and LNCap cells. (f and g) The effect of RNase R on the expression of circGFRA1 and linear form GFRA1 in PC-3 and LNCap. (h and i) Expression of circGFRA1 in PC-3 and LNCap cells analyzed by qRT-PCR after normalization with random primers and oligo (dT) 18 primers. (j and k) The distribution of circGFRA1 in PC-3 and LNCap cells was detected by cellular fragment assay. Data are presented as . , .

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