LMX1B Activated Circular RNA GFRA1 Modulates the Tumorigenic Properties and Immune Escape of Prostate Cancer

Figure 2

Silencing circGFRA1 attenuates the tumorigenic properties and immune escape of PCa. (a and b) Loss-of-function cell models were constructed via transfecting si-NC, si-circGRFA1#1, and si- circGRFA1#2 into PC-3 and LNCap cells. CircGRFA1 and GFRA1 expression were measured by qRT-PCR. (c and d) Cell proliferation was detected by CCK-8. (e and f) EdU assay was performed to evaluate cell proliferation. (g and h) Cell migration was assessed by the transwell assay. (i) VEGF content of PCa cell supernatants. (j) IL-10 content of PCa cell supernatants. (k) TGF-β1 content of PCa cell supernatants. (l and m) The cytotoxicity of CIK cells to PC-3 and LNCap cells was determined via CCK-8 assay. Results from the si-NC group were used as a control. Data are presented as . , , .

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