LMX1B Activated Circular RNA GFRA1 Modulates the Tumorigenic Properties and Immune Escape of Prostate Cancer

Figure 3

LMX1B binds to circGFRA1 and upregulates circGFRA1 expression in PCa cells. (a) Relative circGFRA1 expression in PC-3 and LNCap cells upon 0/20/50/100 ng LMX1B transfection and circGFRA1 expression upon 0 ng LMX1B transfection were used as control. (b) Relative circGFRA1 expression in PCa cells upon si-NC, si-LMX1B#1, and si-LMX1B#2 transfection results from si-NC group was used as control. (c) The predicted binding motif of LMX1B obtained from JASPAR. (d) The predicted binding motif of GFRA1 promoter regions obtained from JASPAR. (e and f) ChIP analysis of enrichment of LMX1B on the GFRA1 promoter in PC-3 and LNCap cells. IgG was used as a negative control. (g and h) Quantification of the luciferase activity of the wild-type or mutant GFRA1 promoter reporter in PC-3 and LNCap cells. Data are presented as . , .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)