LMX1B Activated Circular RNA GFRA1 Modulates the Tumorigenic Properties and Immune Escape of Prostate Cancer

Figure 4

LMX1B is upregulated in PCa and modulates the tumorigenic properties and immune escape of PCa. (a) Relative expression of LMX1B in thirty pairs of PCa tumor tissues and comparative normal tissues. (b) Protein expression of LMX1B in randomly selected five pairs of tumor tissues and comparative normal tissues. (c) Relative expression of LMX1B in PCa cell lines (RWPE-1, DU145, PC-3, LNCap, and 22Rv1 cell lines) with expression in RWPE-1 cells used as the control. (d and e) Loss-of-function cell models were constructed via transfecting si-NC, si-LMX1B#1, and si-LMX1B#2 into PC-3 and LNCap cells, respectively. The mRNA and protein expression of LMX1B were measured by qRT-PCR and western blot. (f and g) Cell proliferation was detected by CCK-8. (h and i) EdU assay to evaluate cell proliferation. (j and k) Cell migration was assessed by the transwell assay. (l) VEGF content of PCa cell supernatants. (m) IL-10 content of PCa cell supernatants. (n) TGF-β1 content of PCa cell supernatants. (o and p) The cytotoxicity of CIK cells to PC-3 and LNCap cells was determined via CCK-8 assay with results from the si-NC group as control. Data are presented as . , , .

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