LMX1B Activated Circular RNA GFRA1 Modulates the Tumorigenic Properties and Immune Escape of Prostate Cancer

<div>CircGFRA1 sponges miR-3064-5p. (a) The enrichment of circGFRA1 in the RISC of PC-3 and LNCap cells using anti-Ago2 or IgG antibody with the IgG group results used as a negative control. (b) Bio-RNA pull-down assay to evaluate the relative enrichment of putative miRNA targets of circGFRA1 in probes in PC-3 and LNCap cells, and bio-NC group was used as control. (c) Relative miR-3064-3p expression in circGFRA1 knockdown cells was determined by qRT-PCR using the si-NC group as control. (d) Schematic of circGFRA1 illustrating the position of the miR-3064-5p binding site. (e) Relative luciferase activity in PC-3 and LNCap cells co-transfected with circGFRA1-WT or circGFRA1-MUT and miR-NC mimic and miR-3064-5p mimic using the NC mimic group results as control. 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Journal of Immunology Research

fig5

Figure 5

Figure 5: LMX1B Activated Circular RNA GFRA1 Modulates the Tumorigenic Properties and Immune Escape of Prostate Cancer 