LMX1B Activated Circular RNA GFRA1 Modulates the Tumorigenic Properties and Immune Escape of Prostate Cancer

Figure 6

MiR-3064-5p targets HECTD1. (a) Schematic illustration of the predicted mRNA targets of miR-3064-5p. (b and c) Biotinylated RNA pull-down using bio-NC and bio-miR-3064-5p probes to assess the relationship between HECTD1 and miR-3064-5p in PC-3 and LNCap cells using the bio-NC group as control. (d) HECTD1 expression in miR-3064-5p overexpressed cells was detected by qRT-PCR using the NC mimic group as control. (e) Schematic of HECTD1 illustrating the position of the miR-3064-5p binding site. (f and g) Relative luciferase activity in PC-3 and LNCap cells co-transfected with HECTD1-WT or HECTD1-MUT and miR-NC mimic, miR-3064-5p mimic in PC-3, and LNCap cells using the NC mimic group as control. (h and i) HECTD1 expression in si-NC, si-circGRFA1#1, and si-circGRFA1#1+miR-3064-5p mimic transfected PC-3 and LNCap cells was measured by qRT-PCR and western blotting using the si-NC group as control. Data are presented as . , .

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