PCSK9 Modulates Macrophage Polarization-Mediated Ventricular Remodeling after Myocardial Infarction

Figure 6

PCSK9 regulated M1 macrophage polarization by targeting TLR4. Representative images of Western blots for TLR4 and downstream MyD88/NF-κB in WT/PCSK9^-/- mouse myocardium after ischemia or sham, . (b) Protein levels of TLR4 and downstream MyD88/NF-κB of (a). (c) Representative images of Western blots for TLR4 and downstream MyD88/NF-κB in LPS/IL4-stimulated RAW264.7 cells after cocultivation with PCSK9 protein for 24 h, . (d) Protein levels of TLR4 and downstream MyD88/NF-κB of (c). (e)TLR4 inhibitor (TAK242) was used to analyze whether TLR4 is involved in PCSK9-regulated macrophage polarization; (f) protein levels of IL6, iNOS, TLR4, and downstream MyD88/NF-κB of (e). ; ; ; ns: not significant.

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