Circular RNA circFGFR1 Functions as an Oncogene in Glioblastoma Cells through Sponging to hsa-miR-224-5p

Figure 4

Hsa-miR-224-5p is necessary for circFGFR1-induced glioma oncogenicity. (a–h) SNB19 cells transfected with mi-NC, hsa-miR-224-5p, inhibitor NC, and inhibitor, respectively. (a) The expression of CXCR4 mRNA in those different groups analyzed by qPCR. (b, c) The expression of CXCR4 protein in those different groups analyzed by western blot. (d, e) The migration ability of those different groups analyzed by Matrigel-free Transwell assay. (f, g) The invasion ability of those different groups analyzed by Matrigel-based Transwell assay. (h) The proliferation ability of those different groups analyzed by CCK8 assay. (i) The expression of hsa-miR-224-5p in WT SNB19 cells and SNB19^miR-/- cells analyzed by qPCR. (j–q) SNB19^miR-/- cells overexpressing Vehicle, circFGFR1, sh-NC, and sh-circFGFR1, respectively, by transducing lentivirus and screening with puromycin. (j) The expression of CXCR4 mRNA in engineered SNB19^miR-/- stable cell lines analyzed by qPCR. (k, l) The expression of CXCR4 protein in engineered SNB19^miR-/- stable cell lines analyzed by western blot. (m, n) The migration ability of t engineered SNB19^miR-/- stable cell lines analyzed by Matrigel-free Transwell assay. (o, p) The invasion ability of engineered SNB19^miR-/- stable cell lines analyzed by Matrigel-based Transwell assay. (q) The proliferation ability of engineered SNB19^miR-/- stable cell lines analyzed by CCK8 assay. The data are presented as the , experiments in (a–q), , , .

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