Deletion of Mettl3 at the Pro-B Stage Marginally Affects B Cell Development and Profibrogenic Activity of B Cells in Liver Fibrosis

Figure 5

Knockout of Mettl3 promotes B cell apoptosis, but barely affects proliferation. B cells were isolated from the spleen of WT and Mettl3 cKO mice using CD19 microbeads and treated with LPS (2 μg/ml), CD40L (200 ng/ml), anti-IgM (1 μg/ml), or TNFa (50 ng/ml). Cells were cultured for 2 days, then collected and subjected to flow cytometry. (a)–(e) Representative flow cytometry plots (a) and quantification (b)–(e) of indicated populations in indicated groups. (f, g) B cells were isolated from the spleen of WT and Mettl3 cKO mice, labeled with CFSE, treated with LPS (5 μg/ml), CD40L (200 ng/ml), anti-IgM (2 μg/ml), or TNFa (50 ng/ml) for 5 days, then collected and subjected to flow cytometry. Representative flow cytometry plots (f) and quantification (g) for cytometry analysis for the proliferation of CD19⁺ splenic B cells in indicated groups. Data in (b)–(e) and (g) were presented as with the indicated significance (; student’s -test).

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