M2-Macrophage-Derived Exosomes Promote Meningioma Progression through TGF-β Signaling Pathway

Figure 2

M2-macrophage-derived exosomes promote meningioma cell proliferation and inhibit apoptosis. (a) Detection of cell viability during 24–72 h proliferation in IOMM-Lee and CH157-MN cells using CCK-8 assay. (b) EdU staining and flow cytometry to detect the fraction of proliferating cells in IOMM-Lee and CH157-MN cells. (c) Western blotting was used to detect the protein expression levels of caspase-3, cleaved-caspase-3, Bcl2, and Bax. (d) FITC-conjugated Annexin V kit was used for labeling apoptotic cells in IOMM-Lee and CH157-MN cells, and distribution was detected by flow cytometry. , , , vs. the control and M0-MDE group.

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